Ischemia preconditioning attenuated myocardial ischemia via upregulating the expression of adiponectin in rat
10.3760/cma.j.issn.0253-3758.2010.10.014
- VernacularTitle:成年大鼠心肌缺血预处理的心肌保护作用及其对脂联素表达的影响
- Author:
Hui WANG
1
;
Jin-Song CHENG
;
Wen-Jing WU
;
Jian-Yan WEN
;
Chang-An YU
;
Wen-Qiang LIAO
;
Wei KONG
;
Yuan-Nan KE
;
Jin-Gang ZHENG
Author Information
1. 北京协和医学院
- Keywords:
Myocardial infarction;
Ischemic preconditioning,myocardial;
Adiponectin
- From:
Chinese Journal of Cardiology
2010;38(10):929-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether adiponectin plays a role in the protection of myocardium in the rat myocardial ischemia preconditioning (IPC) model. Method Infarct size was measured by Masson's Trichrome staining, the expression of protein and mRNA of adiponectin at 0, 6, 12 and 24 h after IPC was examined by immunohistochemistry and quantitative real time RT-PCR, plasma levels of adiponectin at above mentioned four time points after IPC were detected by ELISA in IPC and MI rats. Result Infarct size was smaller in IPC than in MI rats (20% ±2% vs. 31% ±3%, P＜0. 05). The expression of adiponectin mRNA at 6 h and 12 h after IPC was 2. 2 and 2. 1 times higher than in Sham rats at respective time points (P ＜ 0. 05 ). Immunohistochemistry staining evidenced increased adiponectin expression in the ischemic area and weak expression of adiponectin in non-ischemic area ( P ＜ 0. 05 ).Compared to the sham group, the plasma level of adiponectin increased significantly at 0, 6 and 12 h after IPC (0 h:7.40 ±0.47 vs. 10.90 ±1.74;6 h: 8. 18 ±1.41 vs. 10.98 ±1.74;12 h:6. 97±1.02 vs. 9. 31 ±0. 96, P ＜ 0. 05 ). Conclusion IPC reduced infarction size, upregulated the myocardial expression of adiponectin at mRNA and protein levels, and increased plasma adiponectin concentration, suggesting that the adiponecitn may play a critical role in the protective effect of IPC.
