FLK-1 + cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells

VernacularTitle:小鼠胎肝中胎肝激酶-1阳性细胞具有血管祖细胞的功能特性
Author: Cheng-Hao JIN ¹ ; Yoshikazu YONEMITSU ; Wei-Rong DING ; Katsuo SUEISHI
Author Information

1. 江西省人民医院
Keywords: Vascular endothelial growth factor receptor-2; Stem cell; Cell differentiation; Stem cell transplantation
From: Chinese Journal of Cardiology 2010;38(12):1108-1112
CountryChina
Language:Chinese
Abstract: Objective To characterize the differential and proliferative activities of FLK-1+ cells derived from mouse fetal liver. Method The FLK-1+ fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1 + cells into the inferior pole of the spleen of nine-week-old male C57BL/6 mice after two-thirds hepatectomy. Results In response to growth factors, FLK-1 + cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1 + cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver. Conclusion These results indicate that FLK-1 + cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cellbased therapy.