Oxidized low density lipoprotein and peroxisome proliferator-activated receptor α induced endogenous fibroblast growth factor 21 upregulation is protective against apoptosis in cardiac endothelial cells
10.3760/cma.j.issn.0253-3758.2010.12.014
- VernacularTitle:过氧化物酶体增殖物激活受体α诱导内源性成纤维细胞生长因子21表达水平变化对氧化修饰的低密度脂蛋白引起的鼠内皮细胞凋亡的影响
- Author:
Jing-Hua LIU
1
;
Yun L(U)
;
Li-Ke ZHANG
;
Jie DU
;
Xiang-Jun ZENG
;
Gang HAO
;
Dong-Hui ZHAO
;
Guo-Zhong WANG
;
Ying-Chuan ZHANG
Author Information
1. 首都医科大学附属北京安贞医院
- Keywords:
Atherosclerosis;
Fibroblast growth factors;
Lipoproteins,LDL;
Apoptosis
- From:
Chinese Journal of Cardiology
2010;38(12):1113-1117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of peroxisome proliferator-activated receptor (PPAR) α agonist bezafibrate and oxidized low density lipoprotein (ox-LDL) on fibroblast growth factor 21 (FGF21)expression and apoptosis in cardiac endothelial cells. Methods The mRNA level of FGF21 was determined by real time-PCR and the protein concentration of FGF21 in culture media was detected by enzyme-linked immunosorbent assay in cultured cardiac microvascular endothelial cells (CMECs) incubated with 10, 50,100 μg/ml ox-LDL, 50, 100 or 200 μmol/L bezafibrate alone or in combination with 100 μg/ml ox-LDL. CMECs apoptosis in various treatment groups was also determined. Results FGF21 mRNA and protein expressions were significantly upregulated in proportion to increased ox-LDL, and 200 μmol/L bezafibrate alone also significantly upregulated FGF21 expression and CMECs apoptosis was significantly reduced in 200 μ mol/L bezafibrate + 100 μg/ml ox-LDL group compared to 100 μg/ml ox-LDL group( P ＜0. 05 ). Conclusions Our data suggest that bezafibrate and ox-LDL induced upregulation of FGF21 might mediate the protective effect against apoptosis. Endogenous FGF21 could thus play important roles in improving the endothelial function at the early stage of atherosclerosis and slowing the development of coronary heart disease.
