Nifedipine attenuates vascular inflammation via inhibin NF-κB activity.
- Author:
Xin-Yu GAO
1
;
Qin YU
;
Shao-Kui LIU
;
Fa-Qiang LU
;
Su-Min ZHOU
;
Shu-Tao ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Vessels; metabolism; Chemokine CCL2; metabolism; Inflammation; Male; Mice; Mice, Inbred C57BL; NF-kappa B; metabolism; Nifedipine; pharmacology; Vascular Diseases; metabolism
- From: Chinese Journal of Cardiology 2010;38(11):1025-1030
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects and related mechanism of nifedipine on vascular inflammation induced by cuff placement.
METHODSAdult male C57BL/6J mice (10 to 12 weeks of age) were assigned to control (no cuff placement without nifedipine), cuff placement (cuff placement without nifedipine) and treatment (cuff placement with nifedipine 1 or 5 mg×kg(-1)×d(-1)) groups. Activity of NF-κB in injured artery was measured 5 days after operation. MCP-1 expression and nuclear translocation of NF-κB were examined in injured artery 7 days after operation.
RESULTSDNA-binding activity of NF-κB was significantly increased in the injured artery 5 days after cuff placement which could be downregulated by nifedipine 5 mg×kg(-1)×d(-1). MCP-1 mRNA expression in the injured arteries was increased 7 days after cuff placement and which could be significantly attenuated by nifedipine 5 mg×kg(-1)×d(-1). Cuff placement decreased the cytoplasmic level of p50, IκBα, IκBβ, and increased the nuclear level of p50. Nifedipine 5 mg×kg(-1)×d(-1) significantly attenuated these changes.
CONCLUSIONOur results suggest that high dose nifedipine could suppresses expression of MCP-1 induced by injured arteries via the inhibin NF-κB DNA binding activity, thereby attenuating vascular inflammation.
