Nifedipine attenuates vascular inflammation via inhibing NF-κB activity
10.3760/cma.j.issn.0253-3758.2010.11.013
- VernacularTitle:硝苯地平抑制核因子κB活性、改善鼠损伤血管炎症
- Author:
Xin-Yu GAO
1
;
Qin YU
;
Shao-Kui LIU
;
Fa-Qiang LU
;
Su-Min ZHOU
;
Shu-Tao ZHANG
Author Information
1. 大连大学附属中山医院
- Keywords:
Blood vessels;
Inflammation;
Chemokine CCL2;
NF-kappa B
- From:
Chinese Journal of Cardiology
2010;38(11):1025-1030
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and related mechanism of nifedipine on vascular inflammation induced by cuff placement. Methods Adult male C57BL/6J mice ( 10 to 12 weeks of age)were assigned to control ( no cuff placement without nifedipine ) , cuff placement ( cuff placement without ( cuff placement without nifedipine) and treatment (cuff placement with nifedipine 1 or 5 mg · kg-1 · d-1) groups. Activity of NF-κB in injured artery was measured 5 days after operation. MCP-1 expression and nuclear translocation of NF-κB were examined in injured artery 7 days after operation. Results DNA-binding activity of NF-κB was significantly increased in the injured artery 5 days after cuff placement which could be downregulated by nifedipine 5mg · kg-1 · d-1. MCP-1 mRNA expression in the injured arteries was increased 7 days after cuff placement and which could be significantly attenuated by nifedipine 5 mg · kg-1 · d-1. Cuff placement decreased the cytoplasmic level of p50, IκBα, IκBβ, and increased the nuclear level of p50. Nifedipine 5 mg· kg-1 · d-1 significantly attenuated these changes. Conclusion Our results suggest that high dose nifedipine could suppresse expression of MCP-1 induced by injured arteries via the inhibing NF-κB DNA binding activity, thereby attenuating vascular inflammation.
