Establishment of a porcine model of congenital heart defect with decreased pulmonary blood flow
10.3760/cma.j.issn.0253-3758.2011.01.017
- VernacularTitle:Hybrid方法建立肺血减少型先天性心脏病幼猪动物模型
- Author:
Xue-Gang LIU
1
;
Chao SHI
;
Kang-Wu WANG
;
Yi-Yao LIU
;
Gui-Xin DUAN
;
Xiao-Hong LI
;
Wei SONG
;
Jun-Xiang ZHANG
Author Information
1. 蚌埠医学院第一附属医院
- Keywords:
Heart defects,congenital;
Models,animal
- From:
Chinese Journal of Cardiology
2011;39(1):79-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model of congenital heart defect with decreased pulmonary blood flow for better understanding the pathophysiology of pulmonary vascular development and related regulatory mechanisms of congenital heart defect with decreased pulmonary blood flow. Method One to two months old pigs were randomly divided into three groups: control group(group C, n = 6)with right chest small incisions induced transient pulmonary blood reduction;light-moderate stenosis groups (group T1, n = 7):artificial atrial septum defect(ASD)plus controlled pulmonary artery banding to generate a systolic pressure gradient of 20-30 mm Hg(1 mm Hg = 0. 133 kPa);severe stenosis groups (group T2, n = 7): similar surgical procedures as group T1, and controlled pulmonary artery banding to generate a systolic pressure gradient ≥ 30 ～50 mm Hg. 64-slice computed tomography scanning was performed at one month post operation. Arterial blood gas analysis, hemoglobin value, pulmonary vessel,ASD and banding ring diameters and trans-pulmonary artery banding pressure(Trans-PABP)were determined at two months post operation. Results One pig died due to tracheal intubation accident in the C group, one pig died due to bowel obstruction in the T, group and two pigs died due to acute right heart failure and chronic heart failure respectively in T2 group. 64-slice CT angiography results showed that aortic diameter of T1 group was significantly lower than that of C group and banding diameter was significantly lower than aortic diameter in the T1 and T2 groups at one month post operation. Two months after operation, the size of ASD were(8.0 ± 0. 5)mm and(8.9 ± 1.4)mm(P ＞ 0. 05)respectively in the T1 and T2 groups after operation. The Trans-PABP was significantly higher in the T1 and T2 groups than in C group(P ＜0. 01), and the Trans-PABP was significantly higher in the T2 group than in T1 group(P ＜0. 01). PaO2 and SaO2 in the T1 and T2 groups were significantly lower than those in C group. Conclusion Artificial atrial septum defect combined pulmonary artery banding procedures could be successfully used to establish model of congenital heart defect with decreased pulmonary blood flow and this model could help to understand the pathophysiology and monitor therapy efficacy for patients with congenital heart defect with decreased pulmonary blood flow.
