Studies on gene expression and the 5' CpG islands methylation status of E-cadherin in acute myeloid leukemia.
- Author:
Feng GAO
1
;
Yan LI
;
Wei LIU
;
Xiang-lan LU
;
Xia LI
;
Ping-ping WANG
;
Yun-peng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cadherins; genetics; CpG Islands; genetics; DNA Methylation; Gene Expression; Humans; Leukemia, Myeloid, Acute; genetics
- From: Chinese Journal of Hematology 2006;27(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between E-cadherin gene expression and the methylation status of E-cadherin 5' CpG islands in acute myeloid leukemia (AML).
METHODSReverse transcription-PCR (RT-PCR), flow cytometry and methylation specific PCR were used to analyze the E-cadherin gene and protein expression and its 5' CpG island methylation status respectively in bone marrow cells from 55 AML patients and 7 normal controls.
RESULTSAML cells displayed a significant reduction or lack of E-cadherin gene and protein expression, the positive rates were 23.6% and 18.2% (P < 0.01), respectively. All normal bone marrow cells were E-cadherin positive. Thirty-eight of the 55 patients (69.1%) were E-cadherin 5' CpG island methylated whereas the normal controls were completely unmethylated. Twenty-nine of thirty-one (93.5%) E-cadherin-negative samples showed abnormal hypermethylation of the E-cadherin CpG islands.
CONCLUSIONExpression downregulation and methylation of E-cadherin gene in AML suggest that it might be an important event in AML. E-cadherin methylation was associated with the inhibition of E-cadherin gene and protein expression in AML.