The correlated research of acute graft versus host disease after allogeneic stem cell transplantation.
- Author:
Fan YANG
1
;
Hu CHEN
;
Guang-xian LIU
;
Ming LU
;
Kai FENG
;
Ying-ge ZHANG
;
Jing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adult; Fas Ligand Protein; genetics; Female; Gene Expression; Graft vs Host Disease; blood; diagnosis; etiology; Granzymes; genetics; Hematopoietic Stem Cell Transplantation; adverse effects; methods; Humans; Male; Perforin; genetics; Postoperative Complications; blood; diagnosis; etiology; Prognosis; RNA, Messenger; blood; genetics; Reverse Transcriptase Polymerase Chain Reaction; Transplantation, Homologous
- From: Chinese Journal of Hematology 2006;27(4):264-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the relationship between the expression of FasL, Perforin and Granzyme B and the development of acute graft versus host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).
METHODSThe peripheral blood mRNA expression of granzyme B, perforin, fasL from 17 patients after allo-HSCT was detected by competitive quantitative RT-PCR and the relationship between FasL, Granzyme B and Perforin expressions and clinical symptom of aGVHD was analyzed.
RESULTSThe expression level of Granzyme B, Perforin and FasL was 4.6 +/- 0.2, 4.5 +/- 0.1, 1.4 +/- 0.1 before aGVHD occurrence respectively, and was 98.7 +/- 2.5, 91.8 +/- 3.4, 61.5 +/- 2.2, after the occurrence in 14 patients (P < or = 0.05). Over expressions of Granzyme B, Perforin, and FasL during acute GVHD were detected in 13 of 14, 12 of 14, and 12 of 14 patients respectively. The upregulated expressions occurred prior to clinical symptom of aGVHD.
CONCLUSIONThe expressions of Granzyme B, Perforin, and FasL were significantly high in patients with acute aGVHD. Monitoring of the expressions, might predict the occurrence of clinical aGVHD and it severity and prognosis.
