Study of genes involved in chronic myeloid leukemia with t (3; 21) (q26; q22) in blastic crisis.
- Author:
Xu-ping LIU
1
;
Mei-rong ZHANG
;
Yun DAI
;
Li ZHANG
;
Rui LI
;
Yu-shu HAO
;
Zhi-jian XIAO
Author Information
- Publication Type:Case Reports
- MeSH: Adult; Blast Crisis; genetics; pathology; Chromosomes, Human, Pair 21; genetics; Chromosomes, Human, Pair 3; genetics; Core Binding Factor Alpha 2 Subunit; genetics; DNA-Binding Proteins; genetics; Fusion Proteins, bcr-abl; genetics; Genetic Predisposition to Disease; Humans; In Situ Hybridization, Fluorescence; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; pathology; MDS1 and EVI1 Complex Locus Protein; Male; Neoplasm Proteins; genetics; Oncogene Proteins, Fusion; genetics; Proto-Oncogenes; genetics; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; genetics; Translocation, Genetic
- From: Chinese Journal of Hematology 2006;27(5):310-313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore genes involved in chronic myeloid leukemia (CML) with t (3; 21) (q26; q22) chromosome translocation in blastic crisis.
METHODSA case of CML patient with t (3; 21) (q26; q22) in blastic crisis was reported. AML1 and bcr/abl genes were detected by FISH in interphase and metaphase cells. Genes involved in t (3; 21) (q26; q22) were analysed by RT-PCR and sequencing.
RESULTSAML1 gene hybridization signal was detected in der (3) and der (21) chromosomes. AML1-Evi1, AML1-MDS1-Evi1, AML1-EAP fusion transcripts and Evi1 gene were detected in mRNA level, but no AML1-Evi1 fusion transcript. The mRNA expression level of AML1-MDS1-Evi1 fusion gene was 1.58 and 1.54 times higher than that of AML1-MDS1 and AML1-EAP, respectively. The mRNA expression level of Evi1 gene of the patient was 2.71 times higher than that of HEL cell line.
CONCLUSIONt (3; 21) (q26; q22) resulted in the AML1-MDS1-Evi1, AML1-MDS1, AML1-EAP fusion transcripts, and Evi1 gene was also activated by the translocation. These secondary aberrations should be the molecular basis of CML patient with t (3; 21) (q26; q22) in blastic crisis.
