Expression of CD66c (CEACM6) in adult acute leukemia and its significance.
- Author:
Bao-guo CHEN
1
;
Wei-hua YAN
;
Zhe-feng MENG
;
Zong-ming GUO
;
Min ZHU
;
Bo-li LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antigens, CD; biosynthesis; Carcinoembryonic Antigen; biosynthesis; genetics; Cell Adhesion Molecules; biosynthesis; GPI-Linked Proteins; HL-60 Cells; Humans; K562 Cells; Leukemia, Myeloid, Acute; metabolism; Male; Middle Aged; Neoplasm, Residual; metabolism; Precursor Cell Lymphoblastic Leukemia-Lymphoma; metabolism; RNA, Messenger; biosynthesis
- From: Chinese Journal of Hematology 2006;27(6):370-373
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of CD66c (CEACM6) in adult acute leukemia and its significance.
METHODSAcute leukemia cell lines HL-60, K562, LCL721.221 and Jurkat were cultured in vitro. RT-PCR and multi-parameter flow cytometry were applied to analysis of CD66c mRNA and protein expression respectively in the cell lines and patient' s bone marrow leukemic cells. Cytogenetic analysis for 199 bone marrow samples from leukemia patients and Minimal Residual Disease (MRD) detection for 25 CD66c positive B lineage ALL were performed.
RESULTS(1) CD66c expression both on cell surface and in plasma were negative in all the cell lines. (2) Four of 127 AML (3.15%) (mainly of M2 and M4), and 28 of 79 ALL (35.44%) (all of B linage ALL) were CD66c positive the subtypes of the ALL being common B-ALL (20/54) and pre B-ALL (8/11) including 8 Ph + B-linage ALL. (3) Six-month relapse rate was significantly different between the MRD positive and negative patients. (4) CD66c mRNA was strongly expressed in B-linage ALL. For the cell lines, only the HL60 cells weakly expressed CD66c mRNA.
CONCLUSIONCD66c expression could be a useful bio-marker for the MRD analysis in ALL, and is closely associated with its transcription level.
