Study of mutation and single nucleotide polymorphism of PDGFRbeta and SHIP gene in acute myeloid leukemia.
- Author:
Su-jiang ZHANG
1
;
Jian-yong LI
;
Jing-yi SHI
;
Zhan-zhong SHI
;
Bai-wei GU
;
Xue-tao BAI
;
Yong-mei ZHU
;
Zhi-xiang SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Inositol Phosphates; genetics; Leukemia, Myeloid, Acute; genetics; Mass Spectrometry; Mutation; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Receptor, Platelet-Derived Growth Factor beta; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2006;27(6):383-385
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the significance of mutation and single nucleotide polymorphism (SNP) of class III receptor tyrosine kinases such as PDGFRbeta and SHIP in acute myeloid leukemia (AML) patients.
METHODSScreening of the mutation and SNP of PDGFRbeta and SHIP by genomic PCR, RT-PCR, directly sequencing and Mass-ARRAY system was carried out in 273 AML patients.
RESULTSThe mutations of PDGFRbeta R685C and SHIP Q1153L were detected for the first time in AML patients. The positivity ratio was 0.73% and 0.36% respectively.
CONCLUSIONThe mutations of PDGFRbeta R685C and SHIP Q1153L may contribute to leukemogenesis of AML.
