Adriamycin enhances anti-human DR5 monoclonal antibody (mDRA-6) induced HL-60 cells apoptosis.
- Author:
Shu-Lian LI
1
;
Yuan-Fang MA
;
Guang-Chao LIU
;
Jun ZHANG
;
Hui-Ling BAI
;
Ying-Jie LIU
;
Feng LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; pharmacology; Apoptosis; drug effects; Dose-Response Relationship, Drug; Doxorubicin; pharmacology; HL-60 Cells; Humans; Mice; Mice, Inbred BALB C; Receptors, TNF-Related Apoptosis-Inducing Ligand; immunology; TNF-Related Apoptosis-Inducing Ligand; immunology
- From: Chinese Journal of Hematology 2006;27(7):461-464
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate synergistic killing effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody (mDRA-6) and adriamycin(Adr) on HL-60 cells.
METHODSmDRA-6 was prepared by immunizing BALB/c mice with DR5 protein. DR5 expression on Adr-treated HL-60 cells was detected by flow cytometry. Morphologic changes of HL-60 cells were observed under fluorescence microscope. Cytotoxic and apoptotic effects of mDRA-6 and Adr on HL-60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.
RESULTSAdr induce DR5 expression on HL-60 cells. Cell budding, chromatin condensation and apoptotic body formation were observed in HL-60 cells treated by mDRA-6 and Adr. Death and apoptosis of these cells and DNA ladder were exhibited on agarose gel electrophoresis.
CONCLUSIONmDRA-6 and Adr have synergistic killing effect on HL-60 cells.
