Prophylactic effect of matrix metalloproteinase inhibitor on acute graft-versus-host disease in a murine model.
- Author:
Yan GU
1
;
Chuan-fang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Graft vs Host Disease; prevention & control; Hematopoietic Stem Cell Transplantation; adverse effects; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Tissue Inhibitor of Metalloproteinases; therapeutic use
- From: Chinese Journal of Hematology 2006;27(8):515-517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the prophylactic effect of tissue inhibitor of matrix metalloproteinase (TIMP) on acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in a murine model.
METHODSThe murine model of aGVHD after allo-HSCT was established by using female C57BL/b (H-2b) mouse as the donor, and male BALB/c (H-2b) as the recipient. After allo-HSCT, recipient mice were divided into 3 groups. For aGVHD prophylaxis group A was given TIMP (2 mg/d) , group B CsA (5 mg x kg(-1) x d (-1)) was given, and group C nothing. Physical signs, mean survival time (MST), peripheral blood counts and aGVHD histopathology were observed.
RESULTSMice in group C developed typical aGVHD and 100% of mortality, with a MST of 8 days, and those in group A and B had longer survival, the MST being (4.8 +/- 1.4) d and (4.3 +/- 0.9) d respectively, with no statistical difference in peripheral blood count between these two groups. Mice in group A showed less severe signs.
CONCLUSIONSTIMP markedly prolongs MST of allo-HSCT recipients, delays the onset of aGVHD signs, and has no adverse effect on hematopoiesis reconstitution.
