STIM1 gene silencing suppresses tumor formation of human hypopharyngeal carcinoma cell line FaDu in nude mice.
- Author:
Xiaobo CUI
1
;
Yuanhao SUN
;
Shuai WU
;
Jun WANG
;
Yunfei BAI
;
Yaping WANG
;
Boqian WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Gene Silencing; Humans; Hypopharyngeal Neoplasms; pathology; Lentivirus; Membrane Proteins; genetics; Mice; Mice, Nude; Neoplasm Proteins; genetics; Neoplasm Transplantation; RNA, Small Interfering; Real-Time Polymerase Chain Reaction; Stromal Interaction Molecule 1
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2016;51(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the inhibition effect of STIM1 gene silencing on tumor growth of human hypopharyngeal carcinoma cell lines FaDu in nude mice.
METHODSSTIM1 gene in FaDu was silenced by lentiviral infection, and the effect of inhibition was detected by Real-time PCR and Western blot after lentiviral infection. Nude mice were divided into 2 groups, 5 mice in each group. Inhibition group: subcutaneous inject FaDu cells which STIM1 expression was inhibited.
CONTROL GROUPsubcutaneous inject FaDu cells infected with negative control siRNA-expressing lentivirus. Tumor volumes were measured by calipers, and small animal imaging was detected by NightOWL system on the day 10, 14, 18 and 22 after tumor inoculated. Tumor weights were evaluated in the day 22 after tumor inoculated. Statistical analysis was performed using standard student test(P value threshold was 0.05).
RESULTSThe expressions of human STIM1 gene and protein in FaDu cells were suppressed effectively after STIM1-siRNA lentiviral infection. The mean tumor volumes of control group and inhibition group were (51±25) mm3 and (40±35) mm3, respectively, on the day 10, (262±107) and (106±41) mm3 on the day 14, (716±226) and (340±158) mm3 on the day, (1 682±592) mm3 and (917±252)mm3 on the day 22 (P<0.05). On the day 22, the tumor weight was (1.22±0.41) g in control group and (0.66±0.26) g in STIM1-siRNA group (P<0.05). Small animal imaging showed that the tumors had a smaller fluorescence range with lower signal intensity in STIM1-siRNA group than in control group on the day 14, 18 and 22.
CONCLUSIONThe expression of STIM1 in human hypopharyngeal carcinoma cell lines FaDu can be inhibited effectively by lentiviral infection, causing the inhibition of tumor formation and growth.
