Effects of perindopril and enalapril on atherosclerosis development of apolipoprotein E knockout mice
10.3321/j.issn:0253-3758.2008.04.017
- VernacularTitle:培哚普利和依那普利对ApoE基因敲除小鼠动脉粥样硬化进展的影响
- Author:
Quan LU
1
;
Yuan-Nan KE
;
Wen-Li CHENG
;
Yong WANG
;
Chang-An YU
;
Jian-Yan WEN
Author Information
1. 北京协和医学院
- Keywords:
Atherosclerosis;
Angiotensin-converting enzyme inhibitors;
Mice,knockout;
Apolipoproteins E
- From:
Chinese Journal of Cardiology
2008;36(4):350-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of perindopril and enalapril on the development of atherosclemtic lesions in ApoE knockout mice.Metllods ApoE knockout mice were treated with saline/d, n=20)per gavage for 20 weeks.Blood pressure and lipids were measured at the study end.Aortic root atherosclerotic plaque was then quantified and tlle content of collagen and the size of lipid core in the plaque assessed.Cryostat sections were used to quantify the expressions of monocyte/macrophage-2 (MOMA-2),intercellular adhesion molecule-1(ICAM-1),vascular cell adhesion molecule-1(VCAM-1)and matrix metalloproteinases-9(MMP-9)in the plaque by immunofluorescence method.Results Blood pressure and lipid profiles were similar among different groups.Compared with control group,the plaque areas of perindopril group and enalapril group displayed significantly decrease(25.33%and 22.86%,respectively,both P＜0.01).However,no significant difierent were observed in the plaque size between the different ACE inhibitors groups.Perindopril group and enalapril group also significantly decreased the size of lipid core(52.98%and 38.98%.respectively,both P＜0.01)and the expression of MOMA-2(88.38%and 52.16%,respectively,both P＜0.01),ICAM-1(80.87%and 49.59%,respectively,both P＜0.01),VCAM-1(77.56%and 56.44%,respectively,both P＜0.01)and MMP-9(86.93%and 55.56%,respectively,both P＜0.01), and increased the plaque collagen content(298.36%and 168.14%, respectively,both P＜0.01)and the effects of perindopril was superior to those of enalapril(all P＜0.05).Conclusions ACE inhibitors significantly suppressed tissue inflammation and attenuated the development of atherosclerosis in ApoE knockout mice independent of their effects on the lipid and blood pressure.Perindopril is superior to enalapril in stabilizing the plaques and has similar effect on reducing the plaque size as that of enalapril.
