Atrial 11β-hydroxysteroid dehydrogenase type 2 expression in rheumatic heart disease patients with or without atrial fibrillation
10.3321/j.issn:0253-3758.2008.05.001
- VernacularTitle:风湿性心脏病心房颤动患者心房肌11β-羟基类固醇脱氢酶Ⅱ表达研究
- Author:
De-An PEI
1
;
Li LI
;
Zhi-Yun XU
;
Jin-Yu HUANG
;
Zheng-Ming XU
;
Min WANG
Author Information
1. 浙江省杭州市红十字会医院
- Keywords:
Atrial fibrillation;
11β-Hydroxysteroid dehydrogenase Ⅱ;
Remodeling
- From:
Chinese Journal of Cardiology
2008;36(5):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the Mrna and protein expressions of 11β-Hydroxysteroid dehydrogenase type 2(11βHSD2)in patients with atrial fibrillation.Methods Right and left atrial lateral wall tissue samples were obtained during mitral/aortic valve replacement operation from 25 patients with rheumatic heart valve disease(12 in sinus rhythm and 13 in chronic atrial fibrillation).Realtime quantitative PCR and Western blot were used to determine the Mrna and protein expressions of 11βHSD2 in atria specimens.The distribution of 11βHSD2 in human atrial tissue Was analyzed by specific immunohistochemical staining.Echocardiography examination was performed before operation.Results The left atrial diameters were significantly higher in the atrial fibrillation group as compared to sinus rhythm group (P<0.01).Similarly,Mrna expression of 11βHSD2(0.86±0.14 vs 0.33±0.12 in right atria,0.95± 0.15 vs 0.37±0.10 in left atria,all P<0.01)and protein expression of 11βHSD2(1.18±0.64 vs 0.71±0.21 in right atria,P<0.01:and 1.36±0.58 vs 0.85±0.15 in left atria,P<0.05)were also significantly upregulated in atrial fibrillation groups than those in sinus rhythm groups.The mRNA and protein expressions of 11βHSD2 were similar between left atria and right atria both in fibrillation and sinus groups (all P>0.05).The special immunohistochemical staining demonstrated that 11βHSD2 Was abundant in the human atrial myocardium and located mainly in the cytoplasm.Conclusion These findings suggested that upregulated 11βHSD2 might be associated to the development and persistence of atrial fibrillation.
