Effects of C-reactive protein on bone marrow-derived endothelial progenitor cell function
10.3321/j.issn:0253-3758.2008.05.015
- VernacularTitle:C反应蛋白对内皮祖细胞部分生物学功能的影响及其机制的实验研究
- Author:
Bin CUI
1
;
Lan HUANG
;
Hu TAN
;
Xiao-Hui ZHAO
;
Ming-Bao SONG
;
Shi-Yong YU
Author Information
1. 第三军医大学附属新桥医院
- Keywords:
Atherosclerosis;
C-reactive protein;
Stem cells;
Nitric oxide synthase
- From:
Chinese Journal of Cardiology
2008;36(5):435-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of C reactive protein(CRP)on endothelial progenitor cell(EPCs)function.Methods Mononuclear cells(MNCs),isolated from bone marrow by density gradient centrifugation combined with adherent cell filtration,were plated on fibronectin coated culture dishes.After 7 days, adherent cells were cultured with different concentrations of CRP(0,5,10,15,20 μg/ml)for 48 hours.EPCs proliferation and migration ability were observed and adhesion assay Was performed.The eNOS mRNA expression of EPCs were measured by RT-PCR.Results The number of EPCs in CRP groups(10,15,20μg/ml) was obviously lower than that in control group(54±3,47 ±3,39±5 vs.60 ±3,P<0.01).EPCs proliferation capacity was inhibited in CRP groups(10,15,20μg/ml)compared with that in control group(0.297 ±0.036,0.273 ±0.013,0.259 ±0.035 vs.0.345±0.014,P<0.01).EPCs migration capacity was inhibited significantly in CRP groups(5,10,15,20μg/ml)than that in control group(28±2.22±3,19 ±3,16±2 vs.30±2,P<0.05).EPCs adhensive number was lower in CRP groups than that in control group(11±2,9±2,6±2,5±1 vs.12 ±2,P<0.05).The mRNA expressions of eNOS in CRP groups were significantly lower in control group.And compared with control group,NOS activity decreased significantly in CRP groups(10,15,20μg/ml)(57.44±3.25,48.37±3.86,36.82±4.89 vs.68.56 ±2.82,P<0.01).Conclusion CRP could both reduce EPCs number and inhibit EPCs functions.
