AcSDKP attenuates PDGF-induced cardiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway.
- Author:
Li-juan ZHANG
1
;
Wen-dong MA
;
Yan YANG
;
Rui-min WANG
;
Ping CHEN
;
Jing-bo YAN
;
Qian LI
;
Dan-dan LI
;
Fang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; Cells, Cultured; Collagen; metabolism; Fibroblasts; cytology; metabolism; Mitogen-Activated Protein Kinase 1; metabolism; Mitogen-Activated Protein Kinase 3; metabolism; Myocytes, Cardiac; cytology; metabolism; Oligopeptides; metabolism; Phosphorylation; Platelet-Derived Growth Factor; metabolism; Rats; Rats, Wistar; Signal Transduction
- From: Chinese Journal of Cardiology 2008;36(5):444-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2 (ERK1/2) pathway on this process.
METHODSMetabolic activity of fibroblasts was determined by CCK-8. Cell cycle was detected by flow cytometry. Expressions of type I and type III collagen were measured by immunocytochemistry and Western blot. Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot.
RESULT10(-9) mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2, while the protein levels of ERK1/2 were not significantly affected by AcSDKP.
CONCLUSIONAcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.
