AcSDKP attenuates PDGF-induced cadiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway
10.3321/j.issn:0253-3758.2008.05.017
- VernacularTitle:细胞外信号调节激酶1/2在抗纤维化短肽AcSDKP调节大鼠心脏成纤维细胞增殖和胶原表达中的作用
- Author:
Li-Juan ZHANG
1
;
Wen-Dong MA
;
Yan YANG
;
Rui-Min WANG
;
Ping CHEN
;
Jing-Bo YAN
;
Qian LI
;
Dan-Dan LI
;
Fang YANG
Author Information
1. 华北煤炭医学院
- Keywords:
Heart;
Fibrosis;
Extracellular signal-regulated MAP kinase;
Fibroblasts;
Collagen
- From:
Chinese Journal of Cardiology
2008;36(5):444-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2(ERK1/2)pathway on this process.Methods Metabolic activity of fibroblasts was determined by CCK-8.Cell cycle was detected by flow cytometry.Expressions of type Ⅰand type Ⅲ collagen were measured by immunocytochemistry and Western blot.Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot. Result 10-9 mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2.while the protein levels of ERK1/2 were not significantly affected by AcSDKP.Conclusion AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.
