Analysis of heterogeneity in exon 4 of Coxsackievirus and Adenovirus receptor gene in dilated cardiomyopathy

VernacularTitle:扩张型心肌病患者柯萨奇-腺病毒受体外显子4的基因异质性研究
Author: Shuo ZHANG ¹ ; Lei TENG ; Hai-Bo JIA ; Li-Li LI ; Xue-Qin GAO ; Bo YU
Author Information

1. 哈尔滨医科大学附属第二医院
Keywords: Cardiomyopathy,dilated; Enterovirus; Adenoviridae; Genes
From: Chinese Journal of Cardiology 2008;36(6):506-509
CountryChina
Language:Chinese
Abstract: Objective To investigate the genetic pathogenesis of dilated cardiomyopathy (DCM) by examining the heterogeneity of Coxsackievirus binding domain (exon 4) of Adenovirus receptor (CAR) in DCM patients and healthy adults,and to detect possible mutation site in exon 4.Methods Using polymerase chain reaction (PCR),we amplified exon 4 of CAR DNA extracted from blood samples obtained from 50 DCM patients and 40 healthy adults.The PCR products were screened with single-strand conformation polymorphism (SSCP) and then sequenced alternatively based on the SSCP results.Results The segment of CAR exon 4 was successfully amplified and there was no single strand conformational disparity in all samples examined by SSCP.Sequence analysis demonstrated that all amplified sequences of CAR exon 4 from samples of the two groups were identical and there was no genetic heterogeneity of CAR exon 4 between the two groups.Conclusion The genetic heterogeneity of CAR exon 4 might not be responsible for the pathogenesis of DCM.