Hypotensive effects of hydrogen sulfide via attenuating vascular inflammation in spontaneously hypertensive rats
10.3321/j.issn:0253-3758.2008.06.016
- VernacularTitle:硫化氢对自发性高血压大鼠血管炎症反应的调节作用
- Author:
Hong-Fang JIN
1
;
Yan SUN
;
Jia-Min HANG
;
Chao-Shu TANG
;
Jun-Bao DU
Author Information
1. 北京大学第一医院
- Keywords:
Hypertension;
Hydrogen sulfide;
Inflammation;
NF-kappa B
- From:
Chinese Journal of Cardiology
2008;36(6):541-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hydrogen sulfide(H2S)on vascular inflammation and blood pressure in spontaneously hypertensive rats(SHR).Methods Four weeks old male SHR rats were treated with saline(control,n=7),sodium hydrosulfide(NaHS,a H2S donor,n=7)and propargylglycine(PPG,endogenous H2S production inhibitor,n=6) for 5 weeks.Age-natched male Wistar Kyoto(WKY)rats served as normotensive controls(n=8).Five weeks later,systolic blood pressure (SBP) was measured in conscious and quiet rats by means of the standard tail-cuff method.The protein expressions of intereellular adhesive molecule-1(ICAM-1),nuclear transcriptional factor-κB p65(NF-κB p65)and inhibitor of nuclear transcriptional factor-κB(IκB-α) in thoracic aorta of rats were detected by immunohistochemieal assay.while the expression of ICAM-1 mRNA in thoracic aorta of rats were investigated by in situ hybridization.Results SBP of control SHR rats was significantly hisher than that of WIKY rats(P<0.05)accompanied by significantly upregulated expressions of ICAM-1 mRNA,ICAM-1 protein,NF-κB p65 protein in aortic endothelial cells(all P<0.01),while the expression of IkB-a protein in aortic endothelial cells in SHR control group was significantly lower than that of WKY control group(P<0.01).NaHS treated SHR rats showed significantly reduced SBP and downregulated expressions of ICAM-1 mRNA,ICAM-1,NF-κB p65 in aortic endothelial cells and upregulatod expression of IκB-α protein in aortic endothelial cells compared to untreated control SHR rats(all P<0.05).In SHR rats treated with PPG.the expressions of ICAM-l mRNA,ICAM-1 protein,NF-κB p65 protein in aortic endothelial cells were further increased while the expression of IκB-α protein further decreased compared with control SHR rats(all P<0.05).Conclusion H2S might attenuate the development of hypertension through by attenuating vascular inflammation reactions.
