Upregulated voltage-gated potassium channel Kv1.3 on the CD4+ CD28null T lymphocyte from patients with acute coronary syndrome
10.3321/j.issn:0253-3758.2008.07.007
- VernacularTitle:急性冠状动脉综合征患者的CD4+CD28null T淋巴细胞电压门控性Kv1.3钾通道数量增加
- Author:
Shen HUANG
1
;
Jia-Rong TANG
;
Cun-Tai ZHANG
;
Li-Ping SUN
;
Li-Fen GUO
;
Meng SUN
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Coronary disease;
Potassium channel,voltage-gated;
T lymphocyte
- From:
Chinese Journal of Cardiology
2008;36(7):602-607
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of our study is to observe the voltage-gated potassium channel Kv1.3 expression on CD4+CD28null T cells from the peripheral blood of ACS patients by the patch clamp technique.Methods Kv1.3 potassium channels expression from 17 patients with ACS and 11 healthy agematched normal controls was detected in single cell ( CD4+ CD28null T cells and CD4 + CD28 + T cells) by fluorescence microscopy and patch clamp.Results The percent of CD4+ CD28null T cells are higher in the ACS(6.97% ± 2.05% ) than that in the controls ( 1.38% ± 0.84%, P < 0.05 ).The concentration of hsCRP is directly correlated with the number of the CD4+CD28nullT cells in the ACS ( r = 0.52, P < 0.05). The conductance[ (6.89 ± 1.17) nS vs.(3.36 ± 0.66) nS ], dens [ ( 1.95 ± 0.80) n/μm2 vs.( 1.13 ± 0.57) n/μm2] and numbers[ (574.5±97.6) n/cell vs.(280.3±55.3) n/cell] of the Kv1.3 channels on the CD4+ CD28nullT cells are significantly higher than those on the CD4 + CD28 + T cells ( all P < 0.01 ) in ACS patients, but were similar on CD4 + CD28 + T cells between ACS patients and controls.conclusion The CD4+ CD28nullT cells in the ACS and the numbers of Kv1.3 channels on the CD4 + CD28nullT cells from the ACS patients are significantly upregulated and might contribute to the pathogenesis of ACS.
