Establishment of chemiluminescent enzyme immunoassay for detecting antibodies against foot-and-mouth disease virus serotype O in swine.

Chen Cui; Ligang Huang; Jing LI; Xingqi Zou; Yuanyuan Zhu; Lei Xie; Qizu Zhao; Limin Yang; Wenjun Liu

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Establishment of chemiluminescent enzyme immunoassay for detecting antibodies against foot-and-mouth disease virus serotype O in swine.

Author: Chen CUI ¹ ; Ligang HUANG ² ; Jing LI ¹ ; Xingqi ZOU ³ ; Yuanyuan ZHU ³ ; Lei XIE ⁴ ; Qizu ZHAO ³ ; Limin YANG ¹ ; Wenjun LIU ¹
Author Information

1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
2. Department of Clinical Laboratory, First Hospital of Tsinghua University, Beijing 100016, China.
3. China Institute of Veterinary Drug Control, Beijing 100081, China.
4. Beijing Animal Health Inspection Institute, Beijing 100044, China.
Publication Type:Journal Article
Keywords: VP1 protein; chemiluminescent enzyme immunoassay; foot-and-mouth disease virus serotype O
From: Chinese Journal of Biotechnology 2016;32(11):1519-1530
CountryChina
Language:Chinese
Abstract: Recombinant structural protein VP1 of foot-and-mouth disease virus serotype O was expressed in Escherichia coli and then purified using Nickel affinity chromatography. A chemiluminescent enzyme immunoassay (CLEIA) method was established using the purified recombinant protein as coating antigen to detect antibody of foot-and-mouth disease virus serotype O in swine. The specificity of VP1-CLEIA method is 100%. The coefficients of variation in the plate and between plates are 1.10%-6.70% and 0.66%-4.80%, respectively. Comparing with the commercial indirect ELISA kit or liquid phase block ELISA kit, the calculated coincidence rate is 93.50% or 94.00%. The high specificity and stability suggested this detection method can be used to monitor the antibody level of foot-and-mouth disease virus serotype O in swine.