CRISPR-Cas9 mediated genome editing in Caenorhabditis elegans.

Author: Xi'nan MENG ¹ ; Hengda ZHOU ¹ ; Suhong XU ¹
Author Information

1. School of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Publication Type:Journal Article
Keywords: CRISPR-Cas9; NHEJ; genetic screening; genome editing; homology recombination; tissue specific
From: Chinese Journal of Biotechnology 2017;33(10):1693-1699
CountryChina
Language:Chinese
Abstract: The development of genome editing techniques based on CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system has revolutionized biomedical researches. It can be utilized to edit genome sequence in almost any organisms including Caenorhabditis elegans, one of the most convenient and classic genetic model animals. The application of CRISPR-Cas9 mediated genome editing in C. elegans promotes the functional analysis of gene and proteins under many physiological conditions. In this mini-review, we summarized the development of CRISPR-Cas9-based genome editing in C. elegans.