Advances in targeted replacement genome editing in plants.

Author: Honglin WANG ¹ ; Congsheng ZHANG ¹ ; Changlin LIU ¹ ; Chuanxiao XIE ¹
Author Information

1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Publication Type:Journal Article
Keywords: crops genetic improveme; gene function identification; targeted knock-in; targeted replacement
From: Chinese Journal of Biotechnology 2017;33(10):1723-1732
CountryChina
Language:Chinese
Abstract: Targeted replacement genome editing refers to DNA modification and engineering technology that could induce and achieve mutations of targeted gene replacement or knockin at a target gene or DNA region. In this review, the principles, implementation methods, factors that influence efficiency and accuracy, and applications of gene replacement editing were summarized and discussed. It provides the reference for gene functional characterization and genetic improvements through gene replacement strategies in higher plant especially crops.