Production of L-citrulline by a recombinant Corynebacterium crenatum SYPA 5-5 whole-cell biocatalyst.

Author: Qianni LIU ¹ ; Meijuan XU ¹ ; Rongzhen ZHANG ¹ ; Meizhou WANG ¹ ; Xian ZHANG ¹ ; Taowei YANG ¹ ; Zhiming RAO ¹
Author Information

1. Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: Corynebacterium crenatum; L-citrulline; arginine deiminase; whole-cell conversion
From: Chinese Journal of Biotechnology 2017;33(11):1889-1894
CountryChina
Language:Chinese
Abstract: Arginine deiminase (ADI) was first high-efficient expressed in Corynebacterium crenatum SYPA 5-5. The ADI was purified by Ni-NTA affinity chromatography and SDS-PAGE analysis showed the molecular weight (MW) was 46.8 kDa. The optimal temperature and pH of ADI were 37 ℃ and 6.5 respectively. The Michaelis constant was 12.18 mmol/L and the maximum velocity was 0.36 μmol/(min·mL). Under optimal conditions, 300 g/L of arginine was transformed and the productivity reach 8 g/(L·h). The recombinant strain was cultivated in a 5-L fermentor and used for whole-cell transformation of 300 g/L arginine, under repeated-batch bioconversion, the cumulative production reached 1 900 g/L.