Identification and characterization of a taxol-producing endophytic fungus from Taxus media.

Author: Rongfeng FANG ¹ ; Haohao LI ¹ ; Peiji ZHANG ¹ ; Rang CAO ¹ ; Shushen YANG ¹
Author Information

1. College of Life Sciences, Northwest Agriculture & Forestry University, Yangling 712100, Shaanxi, China.
Publication Type:Journal Article
Keywords: 18S rDNA; Aspergillus fumigatus; ITS sequence; Taxus media; endophytic fungus; taxol
From: Chinese Journal of Biotechnology 2017;33(12):1945-1954
CountryChina
Language:Chinese
Abstract: To enrich the resource pool of endophytic fungi from plants which produce taxol, a taxol-producing endophytic fungus TMS-26 was isolated from the stem of Taxus Media. The result of high performance liquid chromatography (HPLC) showed that TMS-26 extract exhibited similar chromatographic peaks and retention time (4.545 min) with authentic taxol. Then mass spectrometry (MS) analysis further confirmed that TMS-26 extracts contained the same mass peaks with authentic taxol ((M+Na)+=876). These indicated that the isolated endophytic fungus TMS-26 can produce taxol. According to the morphological characteristics, the molecular analysis of 18S rDNA and internal transcribed spacer nuclear rDNA gene sequence, the fungus was identified as Aspergillus fumigatus TMS-26.