Whole-cell biotransformation for simultaneous synthesis of L-2-aminobutyric acid and D-gluconic acid in recombinant Escherichia coli.
- Author:
Caizhe ZHANG
1
;
Taowei YANG
1
;
Junping ZHOU
1
;
Junxian ZHENG
1
;
Meijuan XU
1
;
Xian ZHANG
1
;
Zhiming RAO
1
Author Information
- Publication Type:Journal Article
- Keywords: D- gluconic acid; L-2-aminobutyric; dehydratase; dehydrogenase; whole-cell catalyst
- From: Chinese Journal of Biotechnology 2017;33(12):2028-2034
- CountryChina
- Language:Chinese
- Abstract: A whole-cell catalyst using Escherichia coli BL21(DE3) as a host, expressing L- threonine dehydratase from Escherichia coli, and co-expressing leucine dehydrogenase from Bacillus cereus and glucose dehydrogenase from Bacillus subtilis for cofactor regeneration, was constructed and used for one-pot production of L-2-aminobutyric acid (L-ABA) and D- gluconic acid from L-threonine and D-glucose. We used shake-flask culture to study the whole-cell catalytic condition including temperature, pH, proper permeabilization of cells and optimal wet cells amount. Moreover, the whole-cell catalyst was cultured in 5-L fermentor by fed-batch fermentation, and 164 g/L L-threonine and 248 g/L D-glucose were converted to 141.6 g/L L-ABA and 269.4 g/L D-gluconic acid. The whole-cell catalyst is promising to fulfill industrial requirements for L-ABA and D-gluconic acid.
