Recent advances in directed evolution.

Author: Ge QU ¹ ; Jing ZHAO ¹ ; Ping ZHENG ¹ ; Jibin SUN ¹ ; Zhoutong SUN ¹
Author Information

1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Publication Type:Journal Article
Keywords: biocatalysis; directed evolution; saturation mutagenesis; stereoselectivity
From: Chinese Journal of Biotechnology 2018;34(1):1-11
CountryChina
Language:Chinese
Abstract: Screening is the bottleneck of directed evolution. In order to address this problem, a series of novel semi-rational designed strategies have been developed based on combinatorial active-site saturation test and iterative saturation mutagenesis, including single code saturation mutagenesis, double code saturation mutagenesis and triple code saturation mutagenesis. By creation of "small and smart" high qualified mutant libraries and combinatorial mutagenesis of specific sites, these new strategies have been successfully applied in multiparameter optimization, e.g. stereo/regioselectivity and activity. This review summarized recent advances in directed evolution and its applications in biocatalysis field.