Tandem expression and activity determination of antibacterial peptide Spinosan-C from Paa spinosa.

Yue Liu; Zhonggen Zhan; Bing Zhu; Rongquan Zheng; Hongyi Cheng; Zuoming Nie

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Tandem expression and activity determination of antibacterial peptide Spinosan-C from Paa spinosa.

Author: Yue LIU ¹ ; Zhonggen ZHAN ¹ ; Bing ZHU ² ; Rongquan ZHENG ³ ; Hongyi CHENG ³ ; Zuoming NIE ²
Author Information

1. Zhejiang Institute of Economics and Trade, Hangzhou 310018, Zhejiang, China.
2. College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China.
3. Institute of Ecology, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Publication Type:Journal Article
Keywords: Paa spinosa antimicrobial peptide; Spinosan-C; antibacterial activity; specific cleaving; tandem expression
From: Chinese Journal of Biotechnology 2018;34(1):132-139
CountryChina
Language:Chinese
Abstract: Antibacterial peptide can be easily degraded by protease and has the lethal effect on the host Escherichia coli. In order to solve these problems and further improve the expression ability of the Escherichia coli system, the antimicrobial peptide Spinosan-C of Paa spinosa was studied. First, the codon of Spinosan-C was optimized according to E. coli codon usage frequency. Then, the 8 multimeric Spinosan-C gene (8×Spinosan-C) was synthesized and cloned into prokaryotic expression vector pET-28a. The fusion antimicrobial peptide 8×Spinosan-C was further highly expressed in Escherichia coli strain Rosetta. The recombinant 8×Spinosan-C protein was then purified and cleaved specially by formic acid to generate the Spinosan-C monomer. Antibacterial test in vitro suggested that the cleaved Spinosan-C monomer had antibacterial bioactivity against the test bacteria. This study provides a technical reference for the largescale preparation of frog antimicrobial peptides.