Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.
- Author:
Fang LIU
1
;
Jia-Bi WANG
;
Yue-Hua LIU
;
Ya-Gang ZUO
;
Xiao-Hong MAN
Author Information
- Publication Type:Journal Article
- MeSH: Condylomata Acuminata; virology; Female; Human papillomavirus 11; genetics; isolation & purification; Human papillomavirus 6; genetics; isolation & purification; Humans; Male; Microscopy, Fluorescence; Polymerase Chain Reaction
- From: Chinese Medical Sciences Journal 2006;21(2):125-127
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).
METHODSHPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).
RESULTSThe positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.
CONCLUSIONThe sensibility of PCR is superior to that of immunofluorescence.
