Influence of quercetin and x-ray on collagen synthesis of cultured human keloid-derived fibroblasts.
- Author:
Xiao LONG
1
;
Xuan ZENG
;
Fu-quan ZHANG
;
Xiao-jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cells, Cultured; Collagen Type I; biosynthesis; genetics; radiation effects; Collagen Type III; biosynthesis; genetics; radiation effects; Dose-Response Relationship, Drug; Female; Fibroblasts; metabolism; pathology; radiation effects; Humans; Keloid; metabolism; pathology; Male; Quercetin; administration & dosage; pharmacology; RNA, Messenger; biosynthesis; Skin; cytology; Transforming Growth Factor beta1; biosynthesis; genetics; radiation effects
- From: Chinese Medical Sciences Journal 2006;21(3):179-183
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism.
METHODSCollagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression. mRNA expression of collagen I and III, and transforming growth factor (TGF)-beta 1 were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR.
RESULTSQuercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent manner. Immunocytochemical staining indicated that collagen I and III were down-regulated by quercetin and X-ray (P < 0.05), particularly collagen I (P < 0.05). mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group (P < 0.05), especially in the group treated with both quercetin and X-ray (P < 0.01). mRNA level of TGF-beta 1 gene was down-regulated by quercertin (P < 0.05).
CONCLUSIONSQuercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation.
