Role of B lymphocyte and its subpopulations in pathogenesis of immunorelated pancytopenia.
- Author:
Rong FU
1
;
Zong-hong SHAO
;
Hong LIU
;
Yu-hong WU
;
Hua-quan WANG
;
Li-min XING
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; B-Lymphocytes; cytology; immunology; Case-Control Studies; Child; Female; Humans; Lymphocyte Subsets; Male; Middle Aged; Pancytopenia; immunology; pathology
- From: Chinese Medical Sciences Journal 2007;22(3):199-202
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo measure the quantities and apoptosis-related protein levels of B lymphocyte in the patients with immunorelated pancytopenia (IRP) and explore the action of B lymphocyte in the pathogenic mechanism of IRP.
METHODSQuantities of whole B lymphocytes and CD5+ B lymphocytes as well as the expressions of Fas and Bcl-2 in B lymphocytes in 35 patients with untreated IRP, 15 IRP patients in complete remission (CR), and 10 normal controls were assayed by flow cytometry.
RESULTSThe percentages of B lymphocyte and CD5+ B lymphocyte were significantly higher in untreated IRP patients than in CR IRP patients and normal controls (P < 0.05), and there was no significant difference between the latter two groups (P > 0.05). There was no significant difference of Fas expression in B lymphocyte among three groups (P > 0.05). The expression of Bcl-2 in B lymphocyte was significantly higher in untreated patients than in CR patients or normal controls (P < 0.01), and significantly higher in CR patients than in normal controls (P < 0.01). The apoptosis-related index was significantly lower in untreated patients than in CR patients or normal controls (P < 0.05), and significantly lower in CR patients than in normal controls (P < 0.05). The percentage of B lymphocyte was positively correlated with post-treated response time (r = 0.53, P < 0.01).
CONCLUSIONThe production of auto-antibodies in IRP patients probably has some relationship with the abnormal quantities of B lymphocyte and its subpopulations as well as with the inhibition of B lymphocyte apoptosis.