Decreased expression of calcium-sensing receptor involved in the progression of diabetic cardiomyopathy.
- Author:
Zhen JIA
;
Jian SUN
;
Hong-zhu LI
;
Hong-xia LI
;
Xue PENG
;
Hong-jiang SHAO
;
Jin-xia YANG
;
Chang-qing XU
;
Shu-zhi BAI
- Publication Type:Journal Article
- MeSH: Animals; Calcium-Binding Proteins; metabolism; Diabetes Mellitus, Experimental; complications; Diabetes Mellitus, Type 2; Diabetic Cardiomyopathies; metabolism; physiopathology; Disease Progression; Heart; physiopathology; Male; Myocardium; metabolism; pathology; Rats; Rats, Wistar; Receptors, Calcium-Sensing; metabolism; Sarcoplasmic Reticulum Calcium-Transporting ATPases; metabolism; Streptozocin
- From: Chinese Journal of Applied Physiology 2015;31(1):35-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the dynamic expression of calcium-sensing receptor(CaSR) in myocardium of diabetic rats.
METHODSThirty male Wistar rats were randomly divided into 3 groups including control, diabetic-4 week and diabetic-8 week groups(n = 10). The type 2 diabetes mellitus models were established by intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after high-fat and high-sugar diet for one month. The cardiac morphology was observed by electron microscope. Western blot analyzed the expression of CaSR, phospholamban (PLN), a calcium handling regulator, and Ca+-ATPase(SERCA) in cardiac tissues.
RESULTSCompared with control group, the expressions of CaSR and SERCA were decreased, while the expression of PLN was significantly increased in a time-dependent manner in diabetic groups. Meanwhile diabetic rats displayed abnormal cardiac structure.
CONCLUSIONThese results indicate that the CaSR expression of myocardium is reduced in the progression of DCM, and its potential mechanism may be related to the imnaired intracellular calcium homeostasis.