Effect of 5-HT1A receptors in the hippocampal DG on active avoidance learning in rats.
- Author:
Feng-ze JIANG
;
Jing LV
;
Dan WANG
;
Hai-ying JIANG
;
Ying-shun LI
;
Qing-hua JIN
- Publication Type:Journal Article
- MeSH: 8-Hydroxy-2-(di-n-propylamino)tetralin; pharmacology; Animals; Avoidance Learning; Dentate Gyrus; physiology; Piperazines; pharmacology; Pyridines; pharmacology; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT1A; physiology; Serotonin; physiology; Serotonin Receptor Agonists; pharmacology
- From: Chinese Journal of Applied Physiology 2015;31(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of serotonin (5-HTIA) receptors in the hippocampal dentate gyrus (DG) on active avoidance learning in rats.
METHODSTotally 36 SD rats were randomly divided into control group, antagonist group and agonist group(n = 12). Active avoidance learning ability of rats was assessed by the shuttle box. The extracellular concentrations of 5-HT in the DG during active avoidance conditioned reflex were measured by microdialysis and high performance liquid chromatography (HPLC) techniques. Then the antagonist (WAY-100635) or agonist (8-OH-DPAT) of the 5-HT1A receptors were microinjected into the DG region, and the active avoidance learning was measured.
RESULTS(1) During the active avoidance learning, the concentration of 5-HT in the hippocampal DG was significantly increased in the extinction but not establishment in the conditioned reflex, which reached 164.90% ± 26.07% (P <0.05) of basal level. (2) The microinjection of WAY-100635 (an antagonist of 5-HT1A receptor) into the DG did not significantly affect the active avoidance learning. (3) The microinjection of 8-OH-DPAT(an agonist of 5-HT1A receptor) into the DG significantly facilitated the establishment process and inhibited the extinction process during active avoidance conditioned reflex.
CONCLUSIONThe data suggest that activation of 5-HT1A receptors in hipocampal DG may facilitate active avoidance learning and memory in rats.