Expression changes of genes related to calcium pathway in all-trans retinoic acid-induced differentiation of APL cells.
- Author:
Pei-Zheng ZHENG
1
;
Juan HOU
;
Ying-Ping CAO
;
Dong-Hui ZHU
;
Chun-Jun ZHAO
;
Hui-Yong FAN
Author Information
1. Department of Clinical Laboratorial Examination, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, Fujian Province, China. zpzsh@tom.com
- Publication Type:Journal Article
- MeSH:
Calcium;
metabolism;
Cell Differentiation;
drug effects;
Gene Expression Regulation, Leukemic;
Humans;
Leukemia, Promyelocytic, Acute;
genetics;
metabolism;
Signal Transduction;
Tretinoin;
pharmacology;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2010;18(2):282-285
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the role of calcium pathway in myeloid differentiation, the expression level of genes related to calcium pathway in all-trans retinoic acid (ATRA)-induced NB4 cell differentiation was detected by cDNA microarray, some of which were further confirmed by quantitative real time RT-PCR. At the same time, the expressions of these genes in NB4-R1 cells treated with ATRA and 8-CPT-cAM P alone or in combination, and in differentiation of primary cells from ATRA-induced newly diagnosed APL patients were detected by real time RT-PCR. The results showed that during differentiation of ATRA-induced NB4 cells, the expressions of genes related to calcium concentration had changed, the expression of downstream effectors in calcium pathway was up-regulated and confirmed by real time RT-PCR assay. The expression of genes related to calcium concentration did not change significantly when NB4-R1 cells were treated by ATRA or 8-CPT-cAMP alone, but expression changes of those genes were similar to the changes in ATRA-induced NB4 cell differentiation when NB4-R1 cells were treated by ATRA combined with 8-CPT-cAMP. In addition, the expression changes of those genes in ATRA-induced primary cells of patients with APL were also similar to changes in ATRA-induced NB4 cell differentiation. It is concluded that calcium pathway may be involved in ATRA-induced differentiation in APL cell.
