Sensitive Detection of Minimal Residual Neuroblastoma Cells Using RT-PCR for Tyrosine Hydroxylase mRNA.
- Author:
Ki Woong SUNG
1
;
Yun Hee LEE
;
Hye Lim JUNG
;
Hong Hoe KOO
;
Hee Young SHIN
;
Hyo Seop AHN
Author Information
1. Department of Pediatrics, Sungkyunkwan University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Neuroblastoma;
Minimal residual disease;
RT-PCR;
Tyroxine hydroxylase;
Transplantation
- MeSH:
Bone Marrow;
Cell Line;
Diagnosis;
Follow-Up Studies;
Humans;
Neoplasm, Residual;
Neuroblastoma*;
Recurrence;
RNA, Messenger*;
Stem Cells;
Time-to-Treatment;
Tissue Donors;
Transplantation;
Tyrosine 3-Monooxygenase*;
Tyrosine*
- From:Korean Journal of Pediatric Hematology-Oncology
1999;6(1):78-87
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: A sensitive assay to detect minimal residual disease (MRD) in neuroblastoma (NBL) is necessary for accurate assessment of disease status and optimal treatment. Reverse transcriptase-polymerase chain reaction (RT-PCR) for tyrosine hydroxylase (TH) mRNA was developed to detect minimal residual NBL cells. METHODS: Thirteen patients with stage I to IV NBL and one healthy donor were included in this study. Samples for bone marrow (BM) and peripheral blood (PB) were obtained at diagnosis, during treatment, and during follow-up after completion of treatment, and were examined for TH mRNA with RT-PCR. RESULTS: TH mRNA was detected in NBL cell line and cells obtained from BM with cytologic tumor invasion, but was not detected in normal PB mononuclear cells (MNC). Occult NBL cells were detected at a level of 1 for 105~6 normal PB MNC by this method. TH mRNA was detected in 13 of 28 BM or PB samples. TH mRNA came to be not detected after administration of chemotherapeutic agent. TH mRNA was detected in 1 PB sample harvested for transplantation and was detected in 2 of 5 PB samples obtained from patients who were transplanted with autologous PB stem cells in partial response (PR) state. TH mRNA was not detected in patient transplanted in complete response (CR) state. In recent examinations, TH mRNA was detected in 1 of 7 CRs and in 2 of 6 PRs. CONCLUSION: RT-PCR for TH mRNA was a sensitive method to detect minimal residual NBL cells. This method can be used to evaluate initial disease status, response to treatment, time to collect stem cells for transplantation, suitability of cellected stem/progenitor cells and to detect relapse early. But the clinical significances of TH mRNA detected by this method require further investigation.
