Effect of proteasome inhibitor MG-132 on L1210 cell apoptosis and its mechanism.
- Author:
Xiao-Hua LI
1
;
Tian-Yang XUE
;
Wei XU
;
Ji-Zhao GAO
Author Information
1. Department of Pediatrics, Xuzhou Medical College Affiliated Hospital, Xuzhou 221002, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Line, Tumor;
Enzyme Inhibitors;
pharmacology;
Humans;
Leupeptins;
pharmacology;
NF-kappa B;
metabolism
- From:
Journal of Experimental Hematology
2010;18(2):321-325
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of proteasome inhibitor MG-132 on apoptosis of L1210 cells and its mechanism. L1210 cells were treated with MG-132 of different concentrations (0, 2.5, 5, 10, 10 micromol/L). Cell viability was tested by MTT assay, apoptosis rate was detected by using flow cytometry, activity of caspase 3 was detected by colorimetry, the expression of NF-kappaB nuclear protein was detected by Western blot. The results showed that the growth inhibition of L1210 cells treated for a same time (24 hours) was enhanced along with increasing of MG-132 concentrations (0, 2.5, 5, 10, 20 micromol/L); the inhibitory rate, apoptosis rate and activity of caspase 3 increased also along with raising of MG-132 concentrations; while the expression of NF-kappaB nuclear protein decreased along with raising of GM-132 concentrations. It is concluded that MG-132 can induce the apoptosis of L1210. The mechanism of apoptosis may be related to the down-regulation of the expression of NF-kappaB and the activation of caspase 3.
