Expression of NF-kappaB mRNA in acute myeloid leukemia.
- Author:
Min LIU
1
;
Xu-Dong WEI
;
Xiao-Dong LÜ
;
Rui-Hua FAN
;
Qing-Song YIN
;
Jian ZHOU
Author Information
1. Department of Hematology, Henan Tumor Hospital, Zhengzhou 450008, Henan Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Bone Marrow;
metabolism;
Case-Control Studies;
Female;
Humans;
Leukemia, Myeloid, Acute;
genetics;
metabolism;
pathology;
Male;
Middle Aged;
NF-kappa B;
genetics;
metabolism;
RNA, Messenger;
genetics;
Young Adult
- From:
Journal of Experimental Hematology
2010;18(2):359-362
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the expression level of NF-kappaB mRNA in acute myeloid leukemia (AML) using real time fluorescence quantitative polymerase chain reduction (qPCR) detection and to explore the effect of NF-kappaB mRNA in pathogenesis of AML. The fresh bone marrow was collected from 60 newly diagnosed patients with AML, the total RNA was extracted by means of RTIzoL, the cDNA was synthesized, the expression of NF-kappaB mRNA was detected by qPCR using GAPDH as internal reference. 12 normal healthy persons were selected as controls. The results showed that the expression of NF-kappaB mRNA in patients with AML was higher than that in normal healthy persons with significant difference (p<0.05), the expression of NF-kappaB mRNA in patients with AML-M4 and -M5 were higher than that in patients with AML-M1, -M2 and -M3. It is concluded that the expression of NF-kappaB mRNA is higher in patients with AML. The up-regulation of NF-kappaB expression in patients with AML may play an important role in pathogenesis of AML.
