Multiparameter flow cytometric evaluation of bone marrow involvement in B cell non-Hodgkin's lymphoma.
- Author:
Sui-Gui WAN
1
;
Xue-Jing SUN
;
Wu-Han HUI
;
Jing-Juan HE
;
Cong-Yan LIU
;
Hong ZHAO
;
Wan-Ling SUN
;
Li SU
;
Juan XU
Author Information
1. Department of Hematology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Bone Marrow;
pathology;
Female;
Flow Cytometry;
methods;
Humans;
Lymphoma, B-Cell;
pathology;
Lymphoma, Non-Hodgkin;
pathology;
Male;
Middle Aged;
Neoplasm Staging;
Prognosis;
Survival Rate;
Young Adult
- From:
Journal of Experimental Hematology
2010;18(2):473-476
- CountryChina
- Language:Chinese
-
Abstract:
The objective of study was to evaluate the clinical values of multiparameter flow cytometry (MPFC) and cytomorphology of bone marrow aspiration(BMA) in detecting bone marrow involvement in patients with B cell Non-Hodgkin's lymphoma (B-NHL). 96 bone marrow samples from the patients with B-NHL were measured by MPFC using CD45/SSC and CD20/SSC gating strategy combined with anti-kappa and anti-lamda monoclonal antibodies, and then compared with results acquired by cytomorphologic analysis of BMA. The results showed that the bone marrow involvement was confirmed by MPFC in 38 cases (39.6%), while it was detected by cytomorphologic analysis of BMA only in 12 cases (12.5%). There was a significant difference between the two methods (p<0.05). 12 positive cases detected by cytomorphologic analysis of BMA were also positive by MPFC. There was no difference of 3-year overall survival rate between negative and positive cases detected by MPFC, but their 4-year overall survival rate was 73.18+/-6.65% and 44.13%+/-19.55% respectively (p<0.05). It is concluded that the MPFC is a more sensitive method for detecting bone marrow involvement in patients with B-NHL than cytomorphologic analysis of BMA. The 4-year overall survival rate of the patients without bone marrow involvement was significant higher than those of patients with bone marrow involvement. Bone marrow involvement in B-NHL detected by MPFC can be useful for clinical evaluation and prognosis prediction.
