Relationship between asparagine synthetase expression level and cell sensitivity to L-asparaginase in human leukemic cell lines.
- Author:
Ben-Shang LI
1
;
Ying-Yi HE
;
Chang-Ying LUO
;
Hua JIANG
;
Shu-Hong SHEN
;
Li-Min JIANG
;
Bei ZHANG
;
Long-Jun GU
Author Information
1. Department of Hematology/Oncology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
- Publication Type:Journal Article
- MeSH:
Asparaginase;
metabolism;
pharmacology;
Aspartate-Ammonia Ligase;
metabolism;
Cell Line, Tumor;
Humans;
Leukemia;
enzymology
- From:
Journal of Experimental Hematology
2010;18(3):559-563
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to explore the relationship between asparagine synthetase (AsnS) mRNA expression level and the sensitivity of leukemic cell lines to L-asparaginase. The AsnS mRNA expression level in 8 cell lines (Jurkat, HL-60, U937, NB4, THP-1, Namalwa, Karpas299 and K562) was determined by real-time quantitative PCR (RQ-PCR) based on fluorescence dye Eva Green before and after treatment with L-Asp, and the cell proliferation rates were analyzed by CCK-8 assay. The results showed that there was a significant disparity of AsnS expression level in 8 cell lines, and there were significant increases of AsnS expression level in cells co-cultured with L-Asp (p < 0.05). Of all these eight cell lines, cells sensitive to L-asparaginase had lower AsnS expression level and cells resistant to L-asparaginase had higher AsnS expression. U937 which was the most sensitive to L-asparaginase had the lowest AsnS expression level, while K562 was natural resistant to L-asparaginase and possessed of the highest AsnS level. It is concluded that the AsnS plays a critical role in regulating cellular biological behavior after depletion of asparagine, the AsnS mRNA expression level in cells reflects the sensitivity of cells to L-Asp. The results may imply the possibility for the use of L-asparaginase in leukemia with lower AsnS expression level.
