Prognostic significance of telomere length in patients with chronic lymphocytic leukemia.
- Author:
Yan-Qiu HOU
1
;
Wei XU
;
Kou-Rong MIAO
;
Chun QIAO
;
Hua-Yuan ZHU
;
Dan-Xia ZHU
;
Yun ZHUANG
;
Yu-Jie WU
;
Jian-Ning WANG
;
Jian-Yong LI
Author Information
1. Department of Hematology, Nanjing Medical University Second Hospital, Nanjing 210011, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
ADP-ribosyl Cyclase 1;
metabolism;
Adult;
Aged;
Aged, 80 and over;
Case-Control Studies;
Female;
Humans;
Leukemia, Lymphocytic, Chronic, B-Cell;
genetics;
metabolism;
Male;
Middle Aged;
Mutation;
Prognosis;
Telomere;
chemistry;
genetics;
metabolism;
ZAP-70 Protein-Tyrosine Kinase;
metabolism
- From:
Journal of Experimental Hematology
2010;18(3):570-574
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the prognostic significance of telomere length in patients with chronic lymphocytic leukemia (CLL) and to analyze relation of telomere length with Binet stage, IgVH mutation status, CD38, ZAP-70 expression as well as other clinical features. 35 CLL patients who contained 80% or more tumor cells in the peripheral blood or bone marrow samples were selected as objects studied, while 13 healthy donors were served as normal controls. The telomere relative length was detected by using a real-time fluorescent quantitative polymerase chain reaction method (qPCR); the expression of CD38 and ZAP-70 protein were detected by flow cytometry, the IgVH mutation was detected by multiplex PCR. The results showed that the mean telomere relative length in CLL patients and normal controls were 0.384 and 0.443 respectively, but the difference between them was not significant (p > 0.05). The telomere length was significantly correlated with Binet stages and IgVH mutation status. Patients in Binet stage B and C showed significantly shorter telomeres than those in Binet stage A (p = 0.001). Mean telomere relative lengths in patients without IgVH mutation were shorter than those in patients with IgVH mutation (p = 0.015). No relation of telomere length with sex, age, ZAP-70 protein and CD38 were found (p > 0.05). It is concluded that telomere length may have a prognostic significance for CLL patients. Combining telomere length and IgVH mutation status may achieve a better prognostic subclassification for CLL patients.
