Effect of Meisoindigo on Wnt signal pathway in K562 and HL-60 cells.
- Author:
Ming-Xin ZUO
1
;
Yan LI
;
Jian-Hua ZHOU
;
Hong-Bo WANG
;
Xiao-Guang CHEN
Author Information
1. Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Glycogen Synthase Kinase 3;
metabolism;
Glycogen Synthase Kinase 3 beta;
HL-60 Cells;
Humans;
Indoles;
pharmacology;
K562 Cells;
Proto-Oncogene Proteins c-myc;
metabolism;
Signal Transduction;
drug effects;
Wnt Proteins;
metabolism;
beta Catenin;
metabolism
- From:
Journal of Experimental Hematology
2010;18(3):579-582
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the effect of meisoindigo on Wnt signal pathway in K562 cells and HL-60 cells and its possible regulatory mechanism. RT-PCR and Western blot assay were used to detect the expression of GSK-3beta and its downstream associated genes as well as proteins expression respectively. The results showed that the meisoindigo could inhibit the phosphorylation of GSK-3beta and decreased beta-catenin and c-myc genes expression in HL-60 cells, but did not significantly affect the two gene expression in K562 cells. Meisoindigo slightly increased the expression of GSK-3beta protein in HL-60 cells, obviously decreased the expression of p-GSK-3beta and c-MYC proteins in K562 cells and HL-60 cells, while meisoindigo did not affect the expression of beta-catenin in K562 cells significantly, but could decrease the expression of beta-catenin in HL-60 cells. It is concluded that the meisoindigo can affect the Wnt signal pathway through inhibiting the GSK-3beta expression and down-regulating the beta-catenin and c-MYC protein expression, which play an important role in the treatment for chronic myeloid leukemia.
