Effect of bortezomib on sensitization of HL-60 cells to TRAIL.
- Author:
Rong HU
1
;
Rong ZHANG
;
Ying-Chun LI
;
Kun YAO
;
Ying YANG
;
Si-Yuan HOU
;
Wei YANG
;
Zhuo-Gang LIU
Author Information
1. Department of Hematology, Shengjing Hospital, China Medical University, Shenyang 110021, Liaoning Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Boronic Acids;
pharmacology;
Bortezomib;
Caspase 8;
metabolism;
HL-60 Cells;
Humans;
Pyrazines;
pharmacology;
TNF-Related Apoptosis-Inducing Ligand;
pharmacology
- From:
Journal of Experimental Hematology
2010;18(3):617-620
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore whether bortezomib can sensitize HL-60 cells to TNF-related apoptosis-inducing ligand (TRAIL) and to investigate its possible mechanism. The HL-60 cells were treated by different concentrations of TRAIL combined with subtoxic concentration of bortezomib. The proliferative inhibition of treated HL-60 cell was analysed by MTT assay. The cell apoptosis was determined by flow cytometry with Annexin V/PI double staining and the expression of caspase-8 was detected by Western blot. The results showed that the subtoxic concentration of bortezomib combined with 10 ng/ml of TRAIL enhanced apoptosis of HL-60 cells, as compared with TRAIL used alone; the expression of caspase-8 increased correspondingly. It is concluded that subtoxic concentration of bortezomib can sensitize HL-60 cells to TRAIL and its mechanism may be related to upregulation of caspase-8 expression.
