Apoptosis-inducing effect of quercetin and kaempferol on human HL-60 cells and its mechanism.
- Author:
Hui-Juan REN
1
;
Hong-Jun HAO
;
Yong-Jin SHI
;
Xue-Min MENG
;
Yan-Qiu HAN
Author Information
1. Department of Hematology, Inner Mongolian Medical College Affiliated Hospital, Hohhot 010050, Inner Mongolian Autonomous Region, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Cycle;
drug effects;
Cell Proliferation;
drug effects;
Gene Expression Regulation, Leukemic;
HL-60 Cells;
Humans;
Inhibitor of Apoptosis Proteins;
metabolism;
Kaempferols;
pharmacology;
Quercetin;
pharmacology
- From:
Journal of Experimental Hematology
2010;18(3):629-633
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to explore the anti-leukemia effect of quercetin and kaempferol and its mechanism. The HL-60 cells were used as the leukemia models. The inhibitory effects of quercetin and kaempferol on growth of HL-60 cells was assessed by MTT assay. The effect of quercetin and kaempferol on cell cycle in HL-60 cells was detected by flow cytometry. The cytotoxic effect of these 2 drugs was analysed by single cell electrophoresis assay. Western blot analysis was used to study the apoptotic mechanism of HL-60 cells. The results showed that the quercetin and kaempferol had a significant anti-leukemia effect in vitro. The proliferation of HL-60 cells was significantly inhibited in dose-and time-dependent manners after treating with quercetin (r = 0.77) and kaempferol (r = 0.76) respectively, and the cytotoxicity of quercetin was superior to that of kaempferol. The quercetin and kaempferol induced G(2)/M arrest and apoptosis of HL-60 cells. The quercetin and kaempferol could down-regulate the survivin expression. It is concluded that the quercetin and kaempferol have significant anti-leukemia effect in vitro. Furthermore the apoptosis-inducing effect of quercetin is stronger than that of kaempferol, both of which induce apoptosis of HL-60 cells through depressing cell growth, arresting cell cycle and inhibiting expression of survivin.
