Immuno-characterization of mouse model similar to human diffuse large B cell lymphoma.
- Author:
Fang LIU
1
;
Gong ZHANG
;
Xin-Hua ZHOU
;
Jing LIU
;
Yun YUE
;
Tong ZHAO
Author Information
1. Department of Basic Medicine, Medical College, Foshan University of Science and Technology, Foshan 528000, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
B-Lymphocytes;
cytology;
Cell Line, Tumor;
Disease Models, Animal;
Immunophenotyping;
Lymphoma, Large B-Cell, Diffuse;
immunology;
Mice;
Mice, Inbred BALB C;
Mice, Nude
- From:
Journal of Experimental Hematology
2010;18(3):655-659
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to establish the BALB/c mouse model with similar diffuse large B cell lymphoma and to investigate the immuno-characteristics of this model. The experiments were divided into 3 groups including group 1 (BALB/c mice with tumor resulting from injection of A20 cells), group 2 (BALB/c mice without tumor formation resulting from injection of A20 cells) and group 3 (normal BALB/c mice). The CD antigen expression of tumor cells in vitro and in vivo, and the ratios of T/B lymphocytes in peripheral blood and spleen tissue of 3 groups were detected by flow cytometry. The results showed that the animal models were successfully established, the pathologic characteristic of tumor cells from animal model were similar to human diffuse large B cell lymphoma. The positive expression levels of CD3, CD4, CD8, CD19 and CD30 in tumor tissue were (49.27 +/- 23.75)%, (6.07 +/- 3.65)%, (51.2 +/- 23.1)%, (67.06 +/- 16.39)% and (37.93 +/- 17.03)% respectively; as compared with A20 cells, the expression levels of CD3 and CD8 significantly increased, while the expression level of CD19 significantly decreased (p < 0.05). The obvious decrease of CD3 and CD4 expression was observed in peripheral blood of mice with tumor as compared with normal mice (p < 0.05). The expression levels of CD3, CD4 and CD8 decreased while the expression level of CD19 increased in the spleen cells of mice without tumor formation as compared with normal mice (p < 0.05). It is concluded that the immunophenotypes of A20 cells and successfully established animal models can be useful studying human B cell lymphoma.
