Role of Delta-like 1 in differentiation and antigen presentation of mouse bone marrow-derived dendritic cells.
- Author:
Yun-Ru CHEN
1
;
Fan FENG
;
Dan-Dan YIN
;
Ying-Min LIANG
;
Hua HAN
Author Information
1. Department of Hematology, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigen Presentation;
immunology;
Bone Marrow Cells;
cytology;
Cell Differentiation;
immunology;
Cells, Cultured;
Dendritic Cells;
cytology;
immunology;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Intercellular Signaling Peptides and Proteins;
metabolism;
Interleukin-4;
pharmacology;
Male;
Mice;
Mice, Inbred C57BL
- From:
Journal of Experimental Hematology
2010;18(3):704-708
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the role of Delta-like 1 (Dll1) in differentiation and antigen pre-sensation of mouse bone marrow-derived dendritic cells (DCs). In the presence of granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 4 (IL-4), mouse bone marrow cells were co-cultured with OP9-Dll1 and OP9-GFP cell lines respectively. After 8 days, the immature DCs were stimulated with tumor antigen. The surface molecules of the activated DCs including MHC II, CD80 and CD86 were analyzed by flow cytometry. Levels of IL-12 and IL-10 in the culture supernatant were detected by ELISA. In addition, the proliferation of T-cells co-cultured with DCs was analyzed by FACS through mixed T-lymphocyte reaction. The results showed that compared with OP9-GFP, the bone marrow cells co-cultured with OP9-Dll1 produced significantly more CD11c(+) DCs (p < 0.05), and possessed higher levels of surface molecule expression including MHC II, CD80 and CD86 after tumor antigen stimulation. The DCs secreted higher level of IL-12 (p < 0.05) and less IL-10 (p < 0.01). They also resulted in significantly stronger T-cell proliferation response. It is concluded that Dll1 can promote the differentiation of DCs from mouse bone marrow cells and enhance their antigen presentation capacity.
