Spatio-temporal expression study of phosphorylated 70-kDa ribosomal S6 kinase (p70S6k) in mesial temporal lobe epilepsy.
- Author:
Xiao-Liang XING
1
;
Long-Ze SHA
;
Yuan YAO
;
Yan SHEN
;
Li-Wen WU
;
Qi XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Epilepsy, Temporal Lobe; enzymology; Hippocampus; enzymology; Immunohistochemistry; Male; Mice; Mice, Inbred C57BL; Phosphorylation; Ribosomal Protein S6 Kinases, 70-kDa; analysis; metabolism
- From: Chinese Medical Sciences Journal 2012;27(1):7-10
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine the spatio-temporal expression of p70S6k activation in hippocampus in mesial temporal lobe epilepsy.
METHODSTemporal lobe epilepsy model was established by stereotaxically unilateral and intrahippocampal injection of kainite acid (KA) in adult male C57BL/6 mice. Latent and chronic epileptogenesis were represented by mice 5 days after KA injection (n = 5) and mice 5 weeks after KA injection (n = 8), respectively. Control mice (n = 5) were injected with saline. Immunohistochemical assays were performed on brain sections of the mice.
RESULTSHippocampus both ipsilateral and contralateral to the KA injection displayed significantly up-regulated pS6 immunoreactivity in dispersed granule cells in 5-day and 5-week model mice.
CONCLUSIONThe activation of p70S6k is mainly located in the dentate gyrus in KA-induced mouse model of temporal lobe epilepsy, indicating that the activation may be related with the disperse degree and hypertrophy of granule cells.
