Zinc finger protein-activating transcription factor up-regulates vascular endothelial growth factor-a expression in vitro.
- Author:
Lian LI-SHAN
1
,
2
;
Yang YAO-GUO
;
Liu WEI
;
Guo LI-LONG
;
Guan HENG
;
Liu CHANG-WEI
;
Li YONG-JUN
Author Information
- Publication Type:Journal Article
- MeSH: Activating Transcription Factors; physiology; Amino Acid Sequence; Base Sequence; Cells, Cultured; Endothelial Cells; metabolism; Humans; Molecular Sequence Data; Neovascularization, Physiologic; Plasmids; Up-Regulation; Vascular Endothelial Growth Factor A; genetics; Zinc Fingers; physiology
- From: Chinese Medical Sciences Journal 2012;27(3):171-175
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells.
METHODSFirstly, we constructed the ZFP-ATF plasmid, then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot, finally, we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants.
RESULTSThe ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamH1and Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg÷μL, P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165.
CONCLUSIONZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165, which might have beneficial effects in angiogenesis process.
