Comparison between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in vitro.
- Author:
Liang CUI
1
;
Xiao-tong LI
;
Yan-qi YANG
;
Min-kui FU
;
Ding ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cholecalciferol; chemistry; Coculture Techniques; Culture Media; chemistry; Mice; Osteoblasts; cytology; Osteoclasts; cytology; Osteocytes; cytology
- From: Chinese Journal of Stomatology 2010;45(7):411-415
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in co-culture system.
METHODSMLO-Y4 cells and murine osteoblast cells were co-cultured with bone marrow cells with or without vitamin D₃ presence.Bone marrow cells were as control group. Tartrat resistant acid phosphatase (TRAP)+ giant cells with three or more nuclei were counted and compared under a microscope at day 9.
RESULTSIn the absence of vitamin D₃, (1963.3 ± 93.1)/plate osteoclasts were observed when MLO-Y4 cells co-cultured with bone marrow cells in 24-well plate.While only (12.7 ± 5.5)/plate osteoclasts were found in the osteoblast group, and (6.0 ± 1.0)/plate in control group. The statistical difference occurs for any two groups (P < 0.05). Vitamin D₃ could significantly increase osteoclast formation in the three groups.
CONCLUSIONSOsteocytes could induce osteoclastogenesis without the presence of vitamin D₃ and vitamin D₃ could enhance the induction effects of MLO-Y4 and osteoblast cells.
